Coding

Part:BBa_K1321138:Design

Designed by: Gabriella Santosa   Group: iGEM14_Imperial   (2014-10-09)


Metallothionein SmtA with T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The T7 vector was fused to SmtA using the Xba and SpeI site.

Source

Our SmtA was a modification of BBa_K19010, wherein we transformed it into Freiburg format. The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.

References